SpectraMax iD3s and iD5e Multi-Mode Microplate Readers

Item#: SPECTRAMAX iD3s & iD5e

The SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers deliver flexible, scalable solutions for absorbance, fluorescence, and luminescence assays—with advanced capabilities for evolving research needs. The iD3s reader offers a streamlined, affordable platform with a tunable monochromator and temperature control, while the iD5e reader adds enhanced detection modes including time-resolved fluorescence (TRF) and tunable fluorescence polarization (FP) and can be expanded to include TR-FRET, HTRF, BRET and western blot. Both readers support on board fluidics with optional dual injectors.

For live-cell assays and microbial growth experiments, environmental control for CO₂/O₂ regulation and advanced shaking are available to maintain ideal growth conditions. Data acquisition and analysis are driven by SoftMax® Pro Software—the most cited microplate reader software in the industry.

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To see all features and specifications for both iD3s & iD5e models see attached brochure

Supports diverse assay needs: Perform ELISA, nucleic acid and protein quantitation, cell viability, microbial assays, western blot, and more.
Simplified setup & analysis: Over 200 preconfigured protocols and intuitive setup tools help streamline data acquisition and analysis—backed by the industry’s most cited microplate reader software.
Fast, consistent mixing for high-performance flash assays: Dual injectors available for fast kinetic assays with SmartInject® Technology, ensuring complete reagent mixing in every well for high-precision with 10 µL dead volume.
Superior hybrid optical system: The high-efficiency monochromator and filter-based optical system, combined with an ultra-cooled photomultiplier tube (PMT), minimizes noise while increasing sensitivity, selectivity, and dynamic range.
Read height optimization (Z height): Z-height optimization automatically adjusts the detection height to maximize sensitivity and signal quality—while minimizing sample volume—ensuring reliable results even in low-volume or precious samples.
Personalized touchscreen control: The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.